FastQCFastQC Report
Wed 20 May 2026
CR1528_L6647-0003-0016_S58_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1528_L6647-0003-0016_S58_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17893443
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG3309131.849353419573863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG837510.4680541358082958No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCCGTGTAGATC409830.22903920726715368Illumina Single End PCR Primer 1 (96% over 33bp)
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG339750.18987402256793173No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT332420.1857775499103219No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC324970.1816140135802819No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC289800.16195876891887157No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC265710.14849573667851404No Hit
ATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC256810.14352184763994275No Hit
GAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGAC180480.10086376333498254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)