FastQCFastQC Report
Mon 3 Mar 2025
CR1431_S24_trimmed_2P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1431_S24_trimmed_2P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9293523
Sequences flagged as poor quality0
Sequence length36-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314420.3383216461615256No Hit
CAAGCAGAAGACGGCATACGAGATCAGTTCGTCTCGTGGGCTCGGACCGAG118290.12728219427659457RNA PCR Primer, Index 29 (96% over 29bp)
TGCCAGCCACCATGAATATTGCACGGTACCATAAATACTTGACCACCTGTA94180.10133939518953146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)