FastQCFastQC Report
Mon 3 Mar 2025
CR1421_S14_trimmed_1P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1421_S14_trimmed_1P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12679171
Sequences flagged as poor quality0
Sequence length36-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGTTAATCTGGGGGG140130.11051984392354988TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)