FastQCFastQC Report
Mon 3 Mar 2025
CR1420_S13_trimmed_1P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1420_S13_trimmed_1P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16474959
Sequences flagged as poor quality0
Sequence length36-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCT787730.47813776046422934No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCCT559210.3394302832559401No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG325560.1976089894973335No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG282840.17167872769819945No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT238050.14449201360683203No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC202560.12295023010375927No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA198660.12058300114737767No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA194190.11786979257429411No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCCT182620.11084701333702865No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCCT169930.10314441450203304No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT167200.10148735423256591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)