FastQCFastQC Report
Mon 3 Mar 2025
CR1420_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1420_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16662176
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC3061791.8375691146222437No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCT775370.4653473831989291No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCCT538360.32310305688764784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG329500.19775328264447575No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG296060.1776838751433186No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG272490.16353806369588222No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT217080.13028310347940147No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA199730.11987029785305353No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA185980.11161807437395932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)