FastQCFastQC Report
Mon 3 Mar 2025
CR1420_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1420_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16662176
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCT792160.4754240982690376No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCCT566260.33984756852886444No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG328140.19693706272217987No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG284810.17093205593315064No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT239230.14357668530208778No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA204270.12259503200542354No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC203810.1223189576199411No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA195830.11752966719352861No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCCT184390.11066381725892224No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACATCTGGGGGG173420.10408004332687398TruSeq Adapter, Index 7 (97% over 35bp)
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCCT171520.10293973608249006No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT168330.10102522023534022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)