FastQCFastQC Report
Fri 28 Feb 2025
CR1417_S10_trimmed_2P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1417_S10_trimmed_2P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271335
Sequences flagged as poor quality0
Sequence length36-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC902521.2412026127251736No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT232370.31956992766802794No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG207410.2852433562750169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181250.24926646894964954No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA138000.18978633222097455No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA87750.12067935255355446No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA84690.1164710469260459No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCCT81190.11165762545667336No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAG80750.11105250961480938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)