FastQCFastQC Report
Fri 28 Feb 2025
CR1417_S10_trimmed_1P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1417_S10_trimmed_1P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271335
Sequences flagged as poor quality0
Sequence length36-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT251850.34636005630327854No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG225990.3107957479610003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACACTTATCTGGGGGG149000.204914228267574TruSeq Adapter, Index 23 (97% over 41bp)
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA145140.19960571201849453No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA102150.14048314374183007No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA95180.13089755870139388No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCCT91890.12637294252018372No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAG90400.12432380023750797No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT81410.11196018337760534No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCCT79750.1096772463378458No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCCT77580.10669292502683482No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCCT77510.10659665659744737No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCT76600.10534516701541051No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCT75580.10394239847290765No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCT75320.10358483002089712No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCCT72800.1001191665629489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)