FastQCFastQC Report
Fri 28 Feb 2025
CR1410_S3_trimmed_1P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1410_S3_trimmed_1P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13630630
Sequences flagged as poor quality0
Sequence length36-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTTAATCTGGGGGG1054530.7736472928984207TruSeq Adapter, Index 22 (97% over 38bp)
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG253070.18566273165657052No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA206430.1514456778593506No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCCT171450.12578288751143563No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC160770.11794759303128322No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT149000.10931262898339988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)