FastQCFastQC Report
Fri 28 Feb 2025
CR1410_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1410_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13843635
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTTAATCTGGGGGG1079420.7797229557121378TruSeq Adapter, Index 22 (97% over 38bp)
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG255080.18425796403906922No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA207870.1501556491485076No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACTTAATCTGGGGG175560.12681640335070957TruSeq Adapter, Index 22 (97% over 38bp)
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCCT172720.124764919040411No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC161900.11694905275962564No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT149930.10830247980389543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)