FastQCFastQC Report
Fri 28 Feb 2025
CR1409_S2_trimmed_2P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1409_S2_trimmed_2P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11446377
Sequences flagged as poor quality0
Sequence length36-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC1466341.2810516375618242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326700.28541782260011184No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT268110.234231320530505No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG219300.1915890067223891No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA144100.12589136283035235No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG122910.1073789549304553No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA118800.10378829912731338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)