FastQCFastQC Report
Tue 31 Dec 2024
CR1389_17111FL-64-01-06_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1389_17111FL-64-01-06_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20158476
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCCAATCTGGGGG916100.4544490367228158TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCCAATCTGGGG528680.26226188924202404TruSeq Adapter, Index 7 (97% over 39bp)
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC474870.23556840308761434No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC396420.19665177069933265No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC306260.15192616743448265No Hit
TGCCAGCCACCATGAATATTGCACGGTACCATAAATACTTGACCACCTGT227280.1127466183455535No Hit
CCCCTTTTATACTAATAATCTTATAGAAATTTAGGTTAAATACAGACCAA202220.10031512302814956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)