FastQCFastQC Report
Mon 9 Sep 2024
CR1271_17111FL-59-01-52_S170_L004_trimmed_2P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1271_17111FL-59-01-52_S170_L004_trimmed_2P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574837
Sequences flagged as poor quality0
Sequence length36-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG2671631.6118589884172012No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1494700.9017886571071559No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA724910.4373557338753919No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT475370.28680221711984255No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT465800.2810284046835574No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT410930.2479240067338219No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC404050.24377313635120515No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC341490.2060291754302018No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA335270.2022764990087082No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC324140.19556150084613202No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC323310.1950607417738105No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC315370.1902703477566627No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC306100.18467753257543346No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC303470.1830907899727762No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC291730.17600776405825289No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC282410.17038478266784765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270070.1629397622432124No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC269680.16270446581163964No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC269430.16255363476575968No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC262350.15828209954643896No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG256150.15454148960861577No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC227550.13728641795994737No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC221240.13347944236193696No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC214260.12926823956096822No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC206010.12429081504692926No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC205980.12427271532142367No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC200580.12101476473041636No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG200060.12070103615498602No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC199940.12062863725296362No Hit
ATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC195240.11779301359042021No Hit
GAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGAC182910.11035402640762017No Hit
CAAGGTCGCTGTTCAATACATCGGAACGTTCGACCGCGGTCGTACGAGCC179190.10810966044492624No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC178430.10765113406545114No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC173720.10480947716107253No Hit
TGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGA167680.10116539909261249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)