FastQCFastQC Report
Mon 9 Sep 2024
CR1271_17111FL-59-01-52_S170_L004_trimmed_1P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1271_17111FL-59-01-52_S170_L004_trimmed_1P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574837
Sequences flagged as poor quality0
Sequence length36-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1638310.9884320431024449No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA768640.4637391004207161No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT608450.3670925994626674No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT505500.3049803747692964No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC439350.2650704800294567No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT413800.24965554714052393No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC377210.2275799152655317No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA367670.2218242025547521No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC359080.2166416478183164No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC353850.21348626233850745No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC351260.211923652703191No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC339920.2050819564620756No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC334100.20157060971398993No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC333880.20143787839361557No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC329570.19883755116264493No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC329500.19879531846979853No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC317650.19164592689508803No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC311460.1879113501991No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC299600.18075592538255428No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC274930.165871917775119No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC266760.16094275919576162No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG265410.1601282715480098No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC254660.15364253657517113No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC254370.15346757256195037No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC248540.14995019257202952No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC244980.14780235847869877No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC242520.14631818098723987No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC238200.143711820514434No Hit
CAAGGTCGCTGTTCAATACATCGGAGTCGCGAACTATCGTCGATTCGGCC235650.14217334384645833No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC235200.1419018479638744No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC223730.13498171957890145No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC218620.13189873300111488No Hit
CAAGGTCGCTGTTCAATACATCGGAACGTTCGACCGCGGTCGTACGAGCC218310.13171170250422373No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG216150.1304085222678208No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC206360.12450197851116122No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCC192580.11618817126225736No Hit
ATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC185510.11192266928477185No Hit
CAAGGTCGCTGTTCAATACATCGGATCGCGATTACGATGTCGCGCGAGCC178940.10795882939904629No Hit
TGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGA173790.10485170985391892No Hit
GAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGAC172340.10397688978781511No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG170730.10300553785234812No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC167350.10096630211205093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)