FastQCFastQC Report
Mon 9 Sep 2024
CR1264_17111FL-59-01-45_S163_L004_trimmed_2P.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1264_17111FL-59-01-45_S163_L004_trimmed_2P.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16202565
Sequences flagged as poor quality0
Sequence length36-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG7381354.555667574856203No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC6526304.027942489352767No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC4503062.779226622451445No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG3217471.9857781777144545No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA2129621.3143721379917315No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT1670701.0311330335659816No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1512310.9333769066811335No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC1380950.8523033235787049No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT1085270.6698136992507051No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA853980.5270646962379105No Hit
GAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGAC773430.4773503454545623No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG564300.34827818928669624No Hit
TGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGA527850.32578175122272307No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC498710.3077969444961338No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG479490.2959346251658302No Hit
AGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGC455390.28106043703574096No Hit
TGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTT452340.2791780190358749No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC437470.27000045980374093No Hit
TATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAA431230.2661492177318838No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC421820.2603414953126249No Hit
ATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATA403620.2491087059363749No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC389050.24011630257308025No Hit
ATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC383860.23691310604216062No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC333580.20588098242469632No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCTGGAGACT333500.2058316075263392No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC330770.2041466891199017No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC327020.20183224075941064No Hit
TATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTC325320.20078302416932137No Hit
AGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGT314750.19425936572388386No Hit
AGTCTCCAGGCCGAACGGTCGTTTCGCGCGATATCCGATGTATTGAACAGCGACCTTG307040.1895008598947142No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC306670.18927250098981244No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC298020.18393384010494634No Hit
TGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATAC292050.18024923831504455No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC289890.17891611605940172No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC288530.17807674278733027No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC258730.15968459314929456No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC257750.15907975064441957No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC256050.1580305340543303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255070.15742569154945527No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC250000.15429655736607137No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC242120.14943312987789278No Hit
CAAGGTCGCTGTTCAATACATCGGAACGTTCGACCGCGGTCGTACGAGCC240500.14843328818616064No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC239800.14800125782553566No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC236100.1457176687765178No Hit
ACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTAT234100.14448329631758922No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC228280.1408912724621071No Hit
CTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG228220.14085424128833923No Hit
CAAGGTCGCTGTTCAATACATCGGAGTCGCGAACTATCGTCGATTCGGCC225720.13931127571467852No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC224620.1386323708622678No Hit
CAAGGTCGCTGTTCAATACATCGGATCGCGATTACGATGTCGCGCGAGCC223380.13786705993773207No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC220790.13626854760341958No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC219530.1354908929542946No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCC217770.13440464519043743No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCCTGGAGACT209360.1292141090006428No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC207090.12781309625975887No Hit
AGTCTCCAGGCCGCGACGTTAACGCGATATCACTCCGATGTATTGAACAGCGACCTTG206220.12727614424012493No Hit
AGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGC204170.12601091246972315No Hit
TAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCT200870.12397419791249102No Hit
ACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGCCGAAAC179940.11105649012980352No Hit
GGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACG179140.11056274114623209No Hit
CTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGT173240.10692134239239282No Hit
CAAGGTCGCTGTTCAATACATCGGAATCGTACCGCGCGTATCGGTCGGCC171150.10563142317281246No Hit
CAAGGTCGCTGTTCAATACATCGGAACGATTCGACGATCGTCGACGAGCC169910.10486611224827674No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC164610.10159502523211603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)