FastQCFastQC Report
Mon 9 Sep 2024
CR1264_17111FL-59-01-45_S163_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1264_17111FL-59-01-45_S163_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347382
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG7403734.529000423431715No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC7026594.2982968159672295No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC4820282.9486556318314454No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG3225331.9729948195986367No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA2132801.3046737391956706No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT1788441.094022272190128No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1513280.9257017423340324No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC1387250.8486068289099747No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT1088580.6659047913604759No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA855540.5233498550410091No Hit
GAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGAC778590.47627809761832196No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG564100.3450705440173845No Hit
TGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGA531660.325226387931719No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC505460.3091993568144428No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG484340.29627985692143244No Hit
AGGGAGTAATCCCCTTGGCGGTTAAAACGCGGGGGACAGCGCGTACGTGC461970.2825957085972543No Hit
TGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTT454930.2782892086329175No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC441940.2703429821362222No Hit
TATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAA432030.2642808493739242No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC425510.2602924431569532No Hit
ATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATA407100.24903070106271452No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC395530.24195311518382576No Hit
ATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC380700.23288132619645152No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC344580.21078604512942806No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC336350.20575159985861957No Hit
AGTCTCCAGGCCGAACGGTCGTTTCGCGCGATATCCGATGTATTGAACAG335680.20534174829951365No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC332130.20317014675499723No Hit
TATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTC326000.19942031084855058No Hit
AGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGT321910.19691838118176966No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC310530.1899570218644184No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC301520.18444543597256122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301160.18422521722438492No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC297650.18207808442966586No Hit
TGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATAC292900.17917242039122838No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC290350.17761253759164616No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC263130.16096155335453713No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC262230.16041100648409634No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC258650.1582210533772319No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC252580.1545079205954813No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC247040.1511189987485458No Hit
CAAGGTCGCTGTTCAATACATCGGAACGTTCGACCGCGGTCGTACGAGCC244000.14925937376394582No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC243780.1491247956400603No Hit
ACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTAT243230.14878835033034649No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC237610.14535049098381625No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC231500.14161288945226824No Hit
CAAGGTCGCTGTTCAATACATCGGAGTCGCGAACTATCGTCGATTCGGCC229420.14034051446280513No Hit
CAAGGTCGCTGTTCAATACATCGGATCGCGATTACGATGTCGCGCGAGCC229330.14028545977576104No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC228930.14004077227778736No Hit
CTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG228500.13977773321746564No Hit
AGTCTCCAGGCCGCGACGTTAACGCGATATCACTCCGATGTATTGAACAG227620.13923942072192355No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC225460.13791810823286565No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC224970.1376183660478479No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCC221120.13526324887985122No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC210880.12899924893172496No Hit
TAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCT209190.12796544425278616No Hit
AGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGC205950.12598347551919933No Hit
ACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGCCGAAAC181660.11112482720474753No Hit
GGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACG180190.11022560064969425No Hit
CAAGGTCGCTGTTCAATACATCGGAATCGTACCGCGCGTATCGGTCGGCC174600.10680609286551204No Hit
CTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGT174170.10654305380519034No Hit
CAAGGTCGCTGTTCAATACATCGGAACGATTCGACGATCGTCGACGAGCC172750.10567441318738377No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC165940.10150860853438183No Hit
CAAGGTCGCTGTTCAATACATCGGACGATAGTCGCGTCGCACGATCGGCC164450.10059714760442987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)