FastQCFastQC Report
Fri 13 Sep 2024
CR1263_17111FL-59-01-44_S162_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1263_17111FL-59-01-44_S162_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13235921
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAAT188880.14270257430518057No Hit
AGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCG187460.14162973623067107No Hit
AGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAGTGT178000.13448251919907955No Hit
AGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGTGGAGTGCTGC155530.1175059899496227No Hit
AGACCAAGAAGTTATTATCTCGAACTGACACTGAGCCACAACCCAAACAA151070.1141363717719379No Hit
AGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCC138390.10455638107843043No Hit
AGGCGACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCC137980.10424661797241008No Hit
AGGGCTGCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGT137040.10353642938787562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)