FastQCFastQC Report
Fri 13 Sep 2024
CR1249_17111FL-59-01-30_S148_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1249_17111FL-59-01-30_S148_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14672121
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC973250.6633328610089844No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG689850.47017742015622693No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC652510.4447277936162059No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG339900.231663847374214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326200.22232641074865725No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT252710.17223821968207595No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC183680.12518980725417955No Hit
AGTCTCCAGGCCGAACGGTCGTTTCGCGCGATATCCGATGTATTGAACAG167520.11417572142432576No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA148980.10153951156755046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)