FastQCFastQC Report
Mon 10 Jun 2024
CR1142_17111FL-54-01-27_S144_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1142_17111FL-54-01-27_S144_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12092894
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACCACATCTGGGGG176910.1462925251804903TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACACCACATCTAGGGG176110.14563097964804786TruSeq Adapter, Index 1 (97% over 38bp)
TACCACTTGAGGCATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA142890.1181603014133755Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)