FastQCFastQC Report
Fri 7 Jun 2024
CR1132_17111FL-54-01-17_S134_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR1132_17111FL-54-01-17_S134_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8832051
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTAGATCTGGGGG445860.5048204545014516TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTAGATCTGGGG248690.2815767254967164TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTAGATCTGGGGT214070.24237858227947282TruSeq Adapter, Index 7 (97% over 35bp)
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC138120.15638496652702752No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC119620.13543852951030286No Hit
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC91600.10371316922875558Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)