FastQCFastQC Report
Thu 4 May 2023
CR0735_S52_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCR0735_S52_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20464975
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1998010.9763070807562677No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG1236380.6041443979286562No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGC1232740.6023657492862806No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA844610.4127100081969316No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA590660.28861994700702054No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAG557140.272240743025584No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCCT475930.23255830999060592No Hit
CAAGGTCGCTGTTCAATACATCGGAGTCGCGAACTATCGTCGATTCGGCCT434250.2121918057559318No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCCT427640.20896189709491458No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCCT374710.18309819581993136No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCCT372580.1820573931802995No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCCT364490.1781042977086461No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCG361120.17645758179523796No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCCT358100.17498188978975054No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCCT350750.17139038772341525No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCT349560.17080890643648478No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAGC342800.16750570181493016No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCCT333360.16289294269843965No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTA331300.16188634484039194No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCCT330090.16129509075872314No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCT327060.15981451235586655No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCCT311070.15200116296257388No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCCT309030.15100433789926448No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCCT308170.1505841077255164No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCGC305370.14921591646215057No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCCT303040.14807738587513544No Hit
CAAGGTCGCTGTTCAATACATCGGATCGCGATTACGATGTCGCGCGAGCCT302710.14791613476195306No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCCT300610.14688999131442868No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCCT289770.14159313656625527No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCCT286530.14000994381864626No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCCT286500.13999528462653876No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCCT285570.13954084967120656No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCCT283820.1386857301316029No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCCT281110.13736151644455954No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCCT264420.1292061192354254No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCCT261030.12754963052727894No Hit
TATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT260100.1270951955719467No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCCT249920.1221208430501381No Hit
CAAGGTCGCTGTTCAATACATCGGACGATAGTCGCGTCGCACGATCGGCCT242950.11871502408383103No Hit
CAAGGTCGCTGTTCAATACATCGGAACGATTCGACGATCGTCGACGAGCCT238240.11641353092295495No Hit
CAAGGTCGCTGTTCAATACATCGGAACGTTCGACCGCGGTCGTACGAGCCT233880.1142830616699996No Hit
AGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAG232750.1137308987672841No Hit
CAAGGTCGCTGTTCAATACATCGGAATCGTACCGCGCGTATCGGTCGGCCT226810.11082837873000088No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCCT225200.11004166875356555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)